Coding
Part:BBa_K3454046
Designed by: Zhiyu Fang Group: iGEM20_ShanghaiTech_China (2020-10-27)
AsCas12a_6xHis_MBP
This is the coding sequence of AsCas12a with 6x His and MBP tags.This part is the template for AsCas12a extraction and purfication for reaction system.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 1387
Illegal PstI site found at 2449
Illegal PstI site found at 4858
Illegal PstI site found at 5056 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 1387
Illegal PstI site found at 2449
Illegal PstI site found at 4858
Illegal PstI site found at 5056 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 375
Illegal BglII site found at 1571
Illegal BglII site found at 3113
Illegal BglII site found at 3422 - 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 1387
Illegal PstI site found at 2449
Illegal PstI site found at 4858
Illegal PstI site found at 5056 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 1387
Illegal PstI site found at 2449
Illegal PstI site found at 4858
Illegal PstI site found at 5056
Illegal NgoMIV site found at 4657 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 73
[edit]
Categories
Parameters
None |