Coding

Part:BBa_K3454040

Designed by: Ruijie Xiang   Group: iGEM20_ShanghaiTech_China   (2020-10-26)
Revision as of 12:26, 26 October 2020 by Registry (Talk | contribs)

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AsCas12a_RVRR

This part is the template for AsCas12a_E174R/S542R/K548V/N552R extraction and purification for reaction system.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 196
    Illegal PstI site found at 3667
    Illegal PstI site found at 3865
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 886
    Illegal PstI site found at 196
    Illegal PstI site found at 3667
    Illegal PstI site found at 3865
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 3855
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 196
    Illegal PstI site found at 3667
    Illegal PstI site found at 3865
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 196
    Illegal PstI site found at 3667
    Illegal PstI site found at 3865
    Illegal NgoMIV site found at 3399
    Illegal AgeI site found at 2296
    Illegal AgeI site found at 2767
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1039
    Illegal BsaI.rc site found at 2701
    Illegal SapI.rc site found at 289


[edit]
Categories
Parameters
None