Coding

Part:BBa_K3454046

Designed by: Zhiyu Fang   Group: iGEM20_ShanghaiTech_China   (2020-10-27)
Revision as of 20:51, 27 October 2020 by Registry (Talk | contribs)

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AsCas12a_6xHis_MBP

This is the coding sequence of AsCas12a with 6x His and MBP tags.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1387
    Illegal PstI site found at 2449
    Illegal PstI site found at 4858
    Illegal PstI site found at 5056
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1387
    Illegal PstI site found at 2449
    Illegal PstI site found at 4858
    Illegal PstI site found at 5056
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 375
    Illegal BglII site found at 1571
    Illegal BglII site found at 3113
    Illegal BglII site found at 3422
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1387
    Illegal PstI site found at 2449
    Illegal PstI site found at 4858
    Illegal PstI site found at 5056
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1387
    Illegal PstI site found at 2449
    Illegal PstI site found at 4858
    Illegal PstI site found at 5056
    Illegal NgoMIV site found at 4657
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 73


[edit]
Categories
Parameters
None