![](https://parts.igem.org/images/partbypart/icon_coding.png)
Coding
Part:BBa_K3454046
Designed by: Zhiyu Fang Group: iGEM20_ShanghaiTech_China (2020-10-27)
AsCas12a_6xHis_MBP
This is the coding sequence of AsCas12a with 6x His and MBP tags.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 1387
Illegal PstI site found at 2449
Illegal PstI site found at 4858
Illegal PstI site found at 5056 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 1387
Illegal PstI site found at 2449
Illegal PstI site found at 4858
Illegal PstI site found at 5056 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 375
Illegal BglII site found at 1571
Illegal BglII site found at 3113
Illegal BglII site found at 3422 - 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 1387
Illegal PstI site found at 2449
Illegal PstI site found at 4858
Illegal PstI site found at 5056 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 1387
Illegal PstI site found at 2449
Illegal PstI site found at 4858
Illegal PstI site found at 5056
Illegal NgoMIV site found at 4657 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 73
[edit]
Categories
Parameters
None |