Coding

Part:BBa_K3544015

Designed by: Qihan Jin   Group: iGEM20_SCU-China   (2020-10-27)
Revision as of 13:08, 27 October 2020 by Registry (Talk | contribs)

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Improved T2A

T2A is a kind of 2A sequence from Thosea asigna virus, which can relize the cleavage of multiple genes in eukaryotes. It has been proved to achieve bicistronic gene expression in S. cerevisiae by 2020 SCU-China. We add a pair of spacers at the front and back of T2A sequence for better cleavage effciency.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None