Composite
Part:BBa_K3407025:Design
Designed by: Alicia Rodríguez Molina Group: iGEM20_TUDelft (2020-10-23)
pKDsgRNA_4.3 sgRNA targetting phage T7 gene 4.3
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The target sequence (protospacer) is a sequence of 20 nucleotides that must precede a PAM sequence (NGG).
Source
Gibson Assembly.