Composite

Part:BBa_K3407025:Design

Designed by: Alicia Rodríguez Molina   Group: iGEM20_TUDelft   (2020-10-23)
Revision as of 23:21, 23 October 2020 by Registry (Talk | contribs)

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pKDsgRNA_4.3 sgRNA targetting phage T7 gene 4.3


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The target sequence (protospacer) is a sequence of 20 nucleotides that must precede a PAM sequence (NGG).


Source

Gibson Assembly.

References