Composite

Part:BBa_K3645012

Designed by: Huang nan   Group: iGEM20_Peking   (2020-10-26)
Revision as of 03:45, 27 October 2020 by Registry (Talk | contribs)

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ABE

Contains a full circuit with pBAD as a regulatory promoter. Need sgRNA expression for the base editor to function.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
    Illegal NheI site found at 3526
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
    Illegal BamHI site found at 1747
    Illegal BamHI site found at 5805
    Illegal XhoI site found at 2121
    Illegal XhoI site found at 2343
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
    Illegal AgeI site found at 1323
    Illegal AgeI site found at 1457
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


[edit]
Categories
Parameters
None