Composite

Part:BBa_K3628019

Designed by: Tianzuo Zeng   Group: iGEM20_SMS_Shenzhen   (2020-10-27)
Revision as of 12:41, 27 October 2020 by Registry (Talk | contribs)

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T7 promoter-RBS-HpaB-RBS-HpaC

The sequences which express HpaB and HpaC are placed after the T7 promoter. This helps express enzyme of the fermentation system under the regulation of photoswitches.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 47
    Illegal PstI site found at 156
    Illegal PstI site found at 1497
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 156
    Illegal PstI site found at 1497
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 172
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 47
    Illegal PstI site found at 156
    Illegal PstI site found at 1497
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 47
    Illegal PstI site found at 156
    Illegal PstI site found at 1497
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1952


[edit]
Categories
Parameters
None