Composite

Part:BBa_K3332078

Designed by: Meihao Ou   Group: iGEM20_XMU-China   (2020-10-16)
Revision as of 18:13, 27 October 2020 by RuiJie Mo (Talk | contribs)


Formaldehyde promoter-Inverter-MazF-terminator

It can express toxin protein if there is formaldehyde.

Usage and Biology

Formaldehyde promoter provides an inducible scheme for downstream genes and proteins. The inverter plays the role of gene inversion which reverses expression pattern when existing. MazF was designed after formaldehyde promoter and inverter, made sense of our kill switch system in degradation: formaldehyde promoter can be activated after formaldehyde induction and MazF expression is blocked and reversed by inverter. In contrast, without formaldehyde, MazF can express under the pR promoter inside inverter and cause cell death by protein toxicity.

Fig 1. pHCHO_inverter_mazF_B0015

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None