Composite

Part:BBa_K3589210

Designed by: Yannik Schermer   Group: iGEM20_TU_Kaiserslautern   (2020-10-25)
Revision as of 17:46, 25 October 2020 by Yanniks (Talk | contribs)


Level 2 spectinomycin resistance + cCA_marLac_SP20-HA-RGS-8His

This composite part contains a spectinomycin resistance (BBa_K3002102) and the wildtype laccase from an uncultured marine bacteria (BBa_K3589109) fused with a SP20-HA-RGS-8His-tag (BBa_K3589150) for an efficient secretion, easy detection via HA-antibody and purification via nickel-NTA-column.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2401
    Illegal PstI site found at 3795
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2401
    Illegal NheI site found at 2665
    Illegal PstI site found at 3795
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2401
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2401
    Illegal PstI site found at 3795
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2401
    Illegal PstI site found at 3795
    Illegal NgoMIV site found at 1401
    Illegal NgoMIV site found at 1584
    Illegal NgoMIV site found at 1694
    Illegal NgoMIV site found at 3655
    Illegal NgoMIV site found at 4374
    Illegal NgoMIV site found at 4825
    Illegal NgoMIV site found at 5471
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None