Composite

Part:BBa_K3589204

Designed by: Yannik Schermer   Group: iGEM20_TU_Kaiserslautern   (2020-10-25)
Revision as of 17:17, 25 October 2020 by Yanniks (Talk | contribs)


Level 1 Wildtype marLac + cCA + SP20-HA-RGS-8His

This composite part contains the PAR-promoter (BBa_K3002027) in combination with the RPL23-Terminator (BBa_K3002006), cCA secretion signal (BBa_K3002007), SP20-HA-RGS-8His-tag (BBa_K3589150) and the coding sequence of the wildtype laccase from an uncultured marine bacteria (BBa_K3589109) plus the MoClo connectors for positions B1-B2 (BBa_K3002302), B2-B3 (BBa_K3002303), B4-B5 (BBa_K3002304) and B5-B6 (BBa_K3002305).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1379
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 249
    Illegal PstI site found at 1379
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1379
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1379
    Illegal NgoMIV site found at 1239
    Illegal NgoMIV site found at 1958
    Illegal NgoMIV site found at 2409
    Illegal NgoMIV site found at 3055
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None