Composite

Part:BBa_K3589203

Designed by: Yannik Schermer   Group: iGEM20_TU_Kaiserslautern   (2020-10-25)
Revision as of 17:16, 25 October 2020 by Yanniks (Talk | contribs)


Level 1Mutant BaLac + cCA + SP20-HA-RGS-8His

This composite part contains the PAR-promoter (BBa_K3002027) in combination with the RPL23-Terminator (BBa_K3002006), cCA secretion signal (BBa_K3002007), SP20-HA-RGS-8His-tag (BBa_K3589150) and the coding sequence of the mutant laccase from Botrytis aclada (BBa_K3589108) plus the MoClo connectors for positions B1-B2 (BBa_K3002302), B2-B3 (BBa_K3002303), B4-B5 (BBa_K3002304) and B5-B6 (BBa_K3002305).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1170
    Illegal PstI site found at 1257
    Illegal PstI site found at 1321
    Illegal PstI site found at 1693
    Illegal PstI site found at 1981
    Illegal PstI site found at 2058
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 249
    Illegal PstI site found at 1170
    Illegal PstI site found at 1257
    Illegal PstI site found at 1321
    Illegal PstI site found at 1693
    Illegal PstI site found at 1981
    Illegal PstI site found at 2058
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1617
    Illegal BglII site found at 2576
    Illegal BamHI site found at 1956
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1170
    Illegal PstI site found at 1257
    Illegal PstI site found at 1321
    Illegal PstI site found at 1693
    Illegal PstI site found at 1981
    Illegal PstI site found at 2058
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1170
    Illegal PstI site found at 1257
    Illegal PstI site found at 1321
    Illegal PstI site found at 1693
    Illegal PstI site found at 1981
    Illegal PstI site found at 2058
    Illegal NgoMIV site found at 3816
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None