Composite

Part:BBa_K3589202

Designed by: Yannik Schermer   Group: iGEM20_TU_Kaiserslautern   (2020-10-24)
Revision as of 17:14, 25 October 2020 by Yanniks (Talk | contribs)


L1 Wildtype marLac + HA (Phytobrick)

This composite part contains the PAR-promoter (BBa_K3002027) in combination with the RPL23-Terminator (BBa_K3002006), a 3xHA-tag (BBa_K3002017) and the coding sequence of the wildtype laccase from an uncultured marine bacteria (BBa_K3589109) plus the MoClo connectors for positions B2-B3 ( BBa_K3002303), B4-B5 ( BBa_K3002304) and B5-B6 (BBa_K3002305).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1312
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 249
    Illegal PstI site found at 1312
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1312
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1312
    Illegal NgoMIV site found at 1172
    Illegal NgoMIV site found at 1891
    Illegal NgoMIV site found at 2342
    Illegal NgoMIV site found at 2895
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None