Composite
Part:BBa_K3332100
Designed by: Shiyi Zhai Group: iGEM20_XMU-China (2020-10-18)
RNAi sequence of phnF-RNAi sequence of phnJ-J23100-RBS-phnJ-T-J23100-RBS-phnO-T-J23100-RBS-phnE1E2
None
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 438
Illegal NheI site found at 461
Illegal NheI site found at 869
Illegal NheI site found at 892
Illegal NheI site found at 1921
Illegal NotI site found at 1036 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 2134
Illegal XhoI site found at 281
Illegal XhoI site found at 712 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1520
Illegal NgoMIV site found at 1542
Illegal AgeI site found at 4812 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 1532
[edit]
Categories
Parameters
None |