Composite

Part:BBa_K3332097

Designed by: Shiyi Zhai   Group: iGEM20_XMU-China   (2020-10-17)
Revision as of 17:32, 24 October 2020 by Master123 (Talk | contribs)


J23100-B0034-phnJ-B0015-J23100-RBS-phnE1-RBS-phnE2

Subunits of phosphonate ABC transporter, permease protein phnE, from S.meliloti 1021. We use K823004 to construct a new part that can transport glyphosate to cytoplasm. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 1057
    Illegal NheI site found at 2583
    Illegal NheI site found at 2606
    Illegal NotI site found at 2750
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 3234
    Illegal NgoMIV site found at 3256
    Illegal AgeI site found at 2209
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 3246


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