Part:BBa_K3452000
hMT-1A
Literature showed that the absorption capacity of recombinant strain to Cd increased to 7.90 μ mol/mg dry cell weight, which was nearly 19 times higher than that of the control strain. TMCd1 is made up of 156 amino acids, of which 47 are cysteine 2+ residues that can bind 16 Cd. Therefore, Suleman et al. expressed the TMCd1 gene fused to the glutathione S-transferase gene in E. coli BL21 DE3 cells. We used it in the cytoplasm to chelate the cadmium ions inhaled from the cytosol of E. coli.
Contribution
Group: iGEM20_CSU_CHINA (2020-10-26)
Author: Hao Zhou
Summary: We used the SmtA protein constructed by Groningen's team in 2009 to adsorb cadmium ions from outside the cell. We have successfully expressed protein in E. coli DH5α and supplemented experimental data to demonstrate that SmtA can Improve cadmium tolerance of E. coli respectively.（Figure a、b）
Figure b：Growth curve after successful protein expression
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 946
Illegal SpeI site found at 1135
Illegal PstI site found at 1155 - 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 946
Illegal SpeI site found at 1135
Illegal PstI site found at 1155 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 862
Illegal BglII site found at 1051
Illegal BamHI site found at 673 - 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 946
Illegal SpeI site found at 1135
Illegal PstI site found at 1155 - 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 946
Illegal SpeI site found at 1135
Illegal PstI site found at 1155
Illegal AgeI site found at 724
Illegal AgeI site found at 892 - 1000COMPATIBLE WITH RFC[1000]
| None |