Coding

Part:BBa_K3589109

Designed by: Yannik Schermer   Group: iGEM20_TU_Kaiserslautern   (2020-10-23)
Revision as of 14:12, 25 October 2020 by Yanniks (Talk | contribs)


Wildtype laccase from uncultured marine bacteria for Chlamydomonas reinhardtii (Phytobrick)

This basic part contains the coding sequence of a wildtype laccase from an uncultured marine bacteria (B3-B4). Combined with a promoter and a terminator, this basic part should mediate the oxidation of a wide variety of substrates including phenolic compounds and aromatic amines. As this part contains the introns 1-3 of RBCS2, it perfectly matches the part BBa_K3002027 (pAR promoter A1-B2), resulting in a high expression (Eichler-Stahlberg et al., 2009). To detect the target protein a 3xHA-tag from the Kaiser Collection (BBa_K3002017) is recommended. We recommend using the pAR promoter (A1-A3) (BBa_K3002003).
This part was designed to allow synthesis due to a high GC-level.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 817
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 817
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 817
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 817
    Illegal NgoMIV site found at 677
    Illegal NgoMIV site found at 1396
    Illegal NgoMIV site found at 1847
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None