Part:BBa_K3402055

Over-expression UGTB cassette

This device is composed of 50bp-upPXA1(BBa_K3402037), Six site(BBa_K3402032), Pgalk(BBa_K3402033), Rec(BBa_K3402034), Tgki(BBa_K3402019), hph(BBa_K3402012), Ptef1(BBa_K3402007), UGTB(BBa_K3402011), Tsyn7(BBa_K3402001), 50bp-doPXA1(BBa_K3402038).

Usage and Biology

upPXA1 and doPXA1 are homologous arms, which means this device will edit the PXA1 site. The β-Rec/six self-excising system will help to recycle the hygromycin resistance gene. UGTB is the key gene in the synthesis of sophorolipid.
When we add this fragment to the Cas9 expression device, we will use promoters with different expression strength as we characterized before to control the expression level of UGTB. Then we can produce different amount of lactone-type sophorolipids.
When the over-expression of SBLE and UGTB both work in the cell, there will be two type of sophorolipids produced by our engineering yeast, acid type and lactone type. Different types of sophorolipids have different functions. The combination of lactone type and acid type may increase the effect of sophorolipid. So, we can control the ratio of lactone and acid type sophorolipids.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 1076
  Illegal BglII site found at 5893
  Illegal BamHI site found at 3479
  Illegal XhoI site found at 1910
  Illegal XhoI site found at 4631
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 219
  Illegal AgeI site found at 5315
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 5474
  Illegal BsaI.rc site found at 5429