Part:BBa_K3332022

phnC

Subunit of phosphonate ABC transporter, ATP binding protein, from S.meliloti 1021.Use K823004 to construct a new part that can transport glyphosate to cytoplasm.

Biology

Phn system is a gene cluster for organophosphorus transport and degradation in many microorganisms. Sinorhizobium meliloti 1021 encode ABC transporter by phnEE, phnC, phnD gene, this transporter can transport glyphosate to cytoplasm. The phnC gene encodes ATP-binding protein and the phnD encodes phosphonate binding protein of the ABC transporter.

Usage

We ligased the strong promoter (BBa_J23100) and the parts (RBS-phnE1-RBS-phnE2-RBS-phnC-RBS-phnD-Terminator) on the expression vector pSB1C3 by standard assembly. Then the ligation mixture was transformed into E. coli DH5α & E. coli BL21(DE3), enabled the E. coli to transport glyphosate to cytoplasm at higher efficiency.

Characterization

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 112
  Illegal NgoMIV site found at 181
  Illegal NgoMIV site found at 420
  Illegal NgoMIV site found at 424
  Illegal NgoMIV site found at 805
  Illegal NgoMIV site found at 829
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 192
  Illegal SapI.rc site found at 358