Protein_Domain
IMT3_eGFP

Part:BBa_K3484002

Designed by: Andreu Pascuet & Eduard Sune   Group: iGEM20_UPF_Barcelona   (2020-10-26)
Revision as of 12:07, 26 October 2020 by Registry (Talk | contribs)

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Intein-mediated T3(THRB) → eGFP

This part encodes an intein-mediated T3 biosensor with enhanced GFP (eGFP) as reporter (IMT3_eGFP). This part was an intermediate sensor to later on develop the IMT3_sfGFP (BBa_K3484001), visit its page to see the caracteritazationa and final design.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 174
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 664
    Illegal NgoMIV site found at 2167
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1579


[edit]
Categories
//chassis/prokaryote/ecoli
//function/reporter/fluorescence
Parameters
None