Composite

Part:BBa_K3445001

Designed by: Kaleb Ramon   Group: iGEM20_Alma   (2020-10-25)
Revision as of 02:52, 26 October 2020 by Registry (Talk | contribs)

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Estrogen responsive RFP output

This part acts as a estrogen response element controlled inverter. The K123002 piece of the composite part produces the TetR protein, which acts to inhibit the RFP output of the I13521 piece. However, the production of TetR can be blocked by binding of the ERE sequence, therefore allowing the production of RFP.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 635
    Illegal AgeI site found at 747
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None