Coding

Part:BBa_K3589106

Designed by: Yannik Schermer   Group: iGEM20_TU_Kaiserslautern   (2020-10-23)
Revision as of 13:03, 25 October 2020 by Yanniks (Talk | contribs)


Wildtype laccase from uncultured marine bacteria for Escherichia coli


This basic part contains the coding sequence of the wildtype form of a laccase from an uncultured marine bacteria (hereafter referred to as marLac). This part is codon-optimized for Escherichia coli. Combined a promoter and a terminator, this basic part should mediate the oxidation of a wide variety of substrates including phenolic compounds and aromatic amines. This part can be cloned into standard E coli. expression vectors like the commercially available pGEX-6P-1 vector.
This laccase is cold-adapted, thermostable and shows a high tolerance for organic solvents as well as salt (Yang et. al, 2018; doi: 10.3389/fmicb.2018.02998).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 517
    Illegal PstI site found at 22
    Illegal PstI site found at 52
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 517
    Illegal PstI site found at 22
    Illegal PstI site found at 52
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 517
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 517
    Illegal PstI site found at 22
    Illegal PstI site found at 52
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 517
    Illegal PstI site found at 22
    Illegal PstI site found at 52
    Illegal NgoMIV site found at 840
    Illegal AgeI site found at 93
    Illegal AgeI site found at 1105
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None