Composite
Part:BBa_K3407017:Design
Designed by: Alicia Rodríguez Molina Group: iGEM20_TUDelft (2020-10-23)
Cry7Ca1 toxin with inducible T7 promoter, RBS and T7 terminator
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 688
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1388
Illegal XhoI site found at 367 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The Cry protein, Cry7Ca1 (GenBank accession no. EF486523) from the Bt strain BTH-13 is a three-domain Cry protein with a molecular weight of 129,196.50 kDa, whereas the activated toxin has a molecular weight of 68 kDa.
Source
Synthetically synthesised