Composite

Part:BBa_K3407017:Design

Designed by: Alicia Rodríguez Molina   Group: iGEM20_TUDelft   (2020-10-23)
Revision as of 21:50, 23 October 2020 by Registry (Talk | contribs)

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Cry7Ca1 toxin with inducible T7 promoter, RBS and T7 terminator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 688
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1388
    Illegal XhoI site found at 367
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The Cry protein, Cry7Ca1 (GenBank accession no. EF486523) from the Bt strain BTH-13 is a three-domain Cry protein with a molecular weight of 129,196.50 kDa, whereas the activated toxin has a molecular weight of 68 kDa.



Source

Synthetically synthesised

References