Terminator
Part:BBa_K3424001:Design
Designed by: David G Group: iGEM20_UFlorida (2020-10-23)
Revision as of 17:30, 26 October 2020 by DavidMurcia (Talk | contribs)
Plasmid Design Notes
Make sure to check the source paper and type in the sequence of the terminator to see how the authors designed it. If you want to design another gene after this terminator in your plasmid, you should have around 19 intermediate bases before inserting a promoter for your other gene.
Source
Meyer, A.J., Segall-Shapiro, T.H., Glassey, E. et al. Escherichia coli “Marionette” strains with 12 highly optimized small-molecule sensors. Nat Chem Biol 15, 196–204 (2019). https://doi.org/10.1038/s41589-018-0168-3