Coding

Part:BBa_K3468073:Experience

Designed by: Yufei Zhou   Group: iGEM20_TJUSLS_China   (2020-10-20)
Revision as of 08:56, 25 October 2020 by YuanWang (Talk | contribs)


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K3468073

It was predicted by FoldX that DDG decreased, and PyOML was used for visual screening, which did not form hydrogen bonds. after mutating it into ASP, it formed hydrogen bonds with SER-46, which stabilized α1-β1loop and was beneficial to improve the thermal stability of PETase.

Fig.1 Ala47 in PyMOL
Fig.2 A47D in PyMOL

User Reviews

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