Device

Part:BBa_K3402061

Designed by: Yibo Shi   Group: iGEM20_Jiangnan_China   (2020-10-27)
Revision as of 13:44, 27 October 2020 by Registry (Talk | contribs)

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The Cas9 and sgRNA expression cassette

This device is composed of Ptef1 (BBa_K3402007), Cas9 (BBa_K3402023), Tsyn7 (BBa_K3402001) and sgPXA1 (BBa_K3402008).

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 5603
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1730
    Illegal BglII site found at 5294
    Illegal XhoI site found at 1051
    Illegal XhoI site found at 1780
    Illegal XhoI site found at 2143
    Illegal XhoI site found at 5095
    Illegal XhoI site found at 6695
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 5603
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 5603
    Illegal NgoMIV site found at 2557
    Illegal NgoMIV site found at 3661
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1501
    Illegal BsaI site found at 2218


[edit]
Categories
Parameters
None