Generator

Part:BBa_K3552009

Designed by: Liu Xibei   Group: iGEM20_LINKS_China   (2020-10-25)
Revision as of 03:51, 26 October 2020 by Akai-B (Talk | contribs)


GsPilA expressed with DH5a generator

The complete circuit including a GsPilA and its generator with DH5a genes which is the original one we haven’t mutated. This part is in the part collection where we have 13 genes that code for the proteins.

The part collection includes: Parts that are different kinds of type 4 pilus: BBa_K3552003 BBa_K3552004 BBa_K3552005 BBa_K3552006 BBa_K3552007 BBa_K3552008 BBa_K3552018 BBa_K3552019 BBa_K3552020 BBa_K3552021 BBa_K3552022 BBa_K3552023 BBa_K3552024 BBa_K3552025 BBa_K3552026 BBa_K3552027 BBa_K3552028 BBa_K3552029

Our part collection can instruct other teams to designed new rechargeable pilus and substitution of different major pilin.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 4139
    Illegal BglII site found at 5941
    Illegal BamHI site found at 2399
    Illegal BamHI site found at 4464
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 7429
    Illegal AgeI site found at 3328
    Illegal AgeI site found at 5909
    Illegal AgeI site found at 8198
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 9204
    Illegal SapI.rc site found at 5545

Characterization

We expressed this complete circuit plasmid in BL21 and verified the function through Western Blot. We also uses the extracted pili to make batteries and measure the voltage and current.

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Parameters
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