Coding

Part:BBa_K3468041:Experience

Designed by: Yufei Zhou   Group: iGEM20_TJUSLS_China   (2020-10-19)
Revision as of 09:20, 25 October 2020 by YuanWang (Talk | contribs)


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Please enter how you used this part and how it worked out.

Applications of BBa_K3468041

The reduction of DDG was predicted by FoldX. PyOML was used for visual screening, which formed hydrogen bonds with 169-SER and 176-LEU. after mutation to ARG, he formed new hydrogen bonds with 172-ASN, which stabilized α4-β6loop and was beneficial to improve the thermal stability of PETase.

Fig.1 N173R in PyMOL

User Reviews

UNIQ2fba90af2954884d-partinfo-00000000-QINU UNIQ2fba90af2954884d-partinfo-00000001-QINU