Coding

Part:BBa_K3468037:Experience

Designed by: Yufei Zhou   Group: iGEM20_TJUSLS_China   (2020-10-19)
Revision as of 09:03, 25 October 2020 by YuanWang (Talk | contribs)


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Applications of BBa_K3468037

The reduction of DDG was predicted by FoldX. PyOML was used for visual screening, which formed hydrogen bonds with 152-ALA itself and ASP-150. after mutating it into SER, it also formed hydrogen bonds with LYS-177, which stabilized α3-β5loop and α4-β6loop, which was beneficial to improve the thermal stability of PETase.

Fig.1 Ala152 in PyMOL
Fig.2 A152N in PyMOL

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