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Part:BBa_K3365058:Design

Designed by: Zou Aixuan   Group: iGEM20_SJTU-BioX-Shanghai   (2020-10-22)
Revision as of 15:03, 22 October 2020 by Registry (Talk | contribs)

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Fourth potential off-target site for dCas9


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 66
    Illegal NheI site found at 89
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In order to make the same distance between transcription initiation site and target sequence, we add one more base pair to spacer as the lure sequences contain only 20 base pair.


Source

We get this lure sequence from the result of a off-target prediciton model

References