Coding

Part:BBa_K3468027

Designed by: Yufei Zhou   Group: iGEM20_TJUSLS_China   (2020-10-18)
Revision as of 12:32, 27 October 2020 by Dtvnhy (Talk | contribs)


PETase A40P,S54W,A80V,T113P,T116R,Q119D,Q127L,S193R,S214F,K252L,T266P,N275F

The PETase is an enzyme, which can hydrolyze PET and this mutation protein is changed on the basis of the PETase. This protein is changed from A40P,S54W,A80V,T113P,T116R,Q119D,Q127L,S193R,S214F,K252L,T266P,N275F position which can be more stable in higher temperature compared with the wild type.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 240
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 240
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 240
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 240
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 240
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None