Part:BBa_K3447005

xynD, which produces xylanase

T7 promoter is a very specific promoter which is transcribed only by specific T7 RNA polymerase which can activate downstream gene expression after being combined.

Usage and Biology

In our project, we divided T7 RNA polymerase into two parts, respectively called T7_NT (链接) and T7_CT (链接) and attached on the nMag and pMag. Under blue light irradiation, these two parts undergo specific blue light dependent heterodimerization and assemble into complete T7rna polymerase, which acts on PT7 promoter and activates downstream gene expression. And the process is reversible, that is, when blue light is stopped, the dimerized T7RNA polymerase de-dimers and loses its function.

Figure 1. Main principles of the molecular mechanism of T7 promoter

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 24
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design

Design Notes

We added some synonymous mutations to avoid part rules.

Source

We found this sequence data in the following reference.

• Armin Baumschlager, Stephanie K. Aoki, and Mustafa Khammash, Dynamic Blue Light-Inducible T7 RNA Polymerases (Opto-T7RNAPs) for Precise Spatiotemporal Gene Expression Control, ACS Synth. Biol. 2017, 6, 2157-2167.
  
  

References

1. Armin Baumschlager, Stephanie K. Aoki, and Mustafa Khammash, Dynamic Blue Light-Inducible T7 RNA Polymerases (Opto-T7RNAPs) for Precise Spatiotemporal Gene Expression Control, ACS Synth. Biol. 2017, 6, 2157-2167.