Coding

Part:BBa_K3629022

Designed by: Arshia Mostoufi   Group: iGEM20_Calgary   (2020-10-12)
Revision as of 05:02, 26 October 2020 by ArshiaMostoufi (Talk | contribs) (→‎References)


mCitrine codon optimized for Yarrowia lipolytica

mCitrine coding sequence codon-optimized for Yarrowia lipolytica.

Usage and Biology

mCitrine is a monomeric green fluorescent protein that is derived from Aequorea victoria. mCitrine's optimal excitation wavelength is 515nm and its optimal emission wavelength is 529nm (1). mCitrine could be used to tag components and proteins within the cell which can then be visualized using fluorescence spectroscopy and microscopy. See BBa_K3629026 for a functional high expression construct using this coding sequence.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 682
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 647

References

1. [online] https://www.fpbase.org/protein/mcitrine/ (Accessed October 26, 2020)

[edit]
Categories
//awards/part_collection
//cds/reporter/yfp
//chassis/eukaryote/yeast
//classic/reporter
Parameters
None