Generator

Part:BBa_K3524005

Designed by: Kong Yangyang   Group: iGEM20_Nanjing_high-school   (2020-10-18)
Revision as of 09:04, 18 October 2020 by Registry (Talk | contribs)

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NahR-Psal-EGFP

BBa_K3524005 is a bio-sensor (nahR and Psal) for the detection of salicylic acid. We adopted it originally on the chromosome of Pseudomonas Stutzeri. With the appearance of salicylic acid, the protein translated from nahR binds with the Psal promoter and initiates the expression of the enzyme that can utilize the acid as one of the energy sources for Pseudomonas Stutzeri. In our design, we replaced the essential gene with the GFP gene to test our framework. With GFP, the situation is that the edited bacteria can be fluorescent with the existence of salicylic acid.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 373
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 541
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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