Composite

Part:BBa_K3332064

Designed by: Yuan Li   Group: iGEM20_XMU-China   (2020-10-17)
Revision as of 19:47, 17 October 2020 by Registry (Talk | contribs)

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J23100-RBS-GFP-BrkA-terminator

This is an anchor proteins onto membranes through BrkA and use GFP to comformation. We use K880005 and GFP to verify BrkA's function which can anchor proteins onto membranes.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1088
    Illegal NgoMIV site found at 1517
    Illegal NgoMIV site found at 2153
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 705
    Illegal BsaI.rc site found at 2121


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Categories
Parameters
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