![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K3332064
Designed by: Yuan Li Group: iGEM20_XMU-China (2020-10-17)
J23100-RBS-GFP-BrkA-terminator
This is an anchor proteins onto membranes through BrkA and use GFP to comformation. We use K880005 and GFP to verify BrkA's function which can anchor proteins onto membranes.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1088
Illegal NgoMIV site found at 1517
Illegal NgoMIV site found at 2153 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 705
Illegal BsaI.rc site found at 2121
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Categories
Parameters
None |