Coding

Part:BBa_K3406001:Design

Designed by: Xiaojie Zhou   Group: iGEM20_ZJUT_China_B   (2020-08-16)
Revision as of 07:38, 16 August 2020 by Registry (Talk | contribs)

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CcaCas13b coding sequence


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 954
    Illegal AgeI site found at 1806
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence of Cas13 protein contains rare codens,so in order to be successfully expressed,host which is is enriched in tRNAs for rare codons is required(such as E.coli Rosetta2).In addition,Cas13 protein might be toxic to the host,so pLysS is also needed to reduce it's basic expression.Meanwhile,this protein is not very soluble and should be tagged with a solubility tag, such as SUMO or MBP.


Source

Capnocytophaga canimorsus

References