Part:BBa_K3595007

Mutant cysE-11-2

cysE-11-2 is a mutant of cysE gene, which is obtained by substituting CGT (Arg), ACC (Thr) in 89th, 90th positions into CAT (His), GTA (Val)), and substituting CCG (Pro), GCA (Ala) in 93th and 94th position into GCT (Ala), ACA (Thr).In the pathway of converting hydrogen sulfide to L-cysteine, L-serine and Acetyl-CoA form O-acetylserine catalyzed by L-serine O-acetyltransferase (SAT). SAT is encoded by gene cysE, and its feedback is inhibited by the final product L-cysteine . In order to induce overproduction of L-cysteine, we need to develop a mutant gene of cysE which will code for feedback inhibition-insensitive SAT.We tried to construct different mutants through site mutation, and cysE-11-2 is one of the different mutants.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 684
  
• 1000
  COMPATIBLE WITH RFC[1000]