Composite

Part:BBa_K3584008

Designed by: Kong Yangyang   Group: iGEM20_Shanghai_United   (2020-10-17)
Revision as of 13:33, 17 October 2020 by Registry (Talk | contribs)

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p14 pro-rbs-tyrR-rbs-tyrP-PPO

In Escherichia coli, the TyrR protein can both repress and activate the transcription operons required for the biosynthesis and uptake of aromatic amino acids (tyrosine, phenylalanine, and tryptophan). In BBa_K3584008, the promoter of the tyrP gene (encodes a tyrosine-specific transporter) is activated by the TyrR dimer in the presence of tyrosine. Then, the polyphenol oxidase(PPO) gene is expressing.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 798
    Illegal BglII site found at 1140
    Illegal BglII site found at 3083
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 604
    Illegal AgeI site found at 2035
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None