DNA

Part:BBa_K3593001

Designed by: Yiming Dong   Group: iGEM20_GreatBay_SCIE   (2020-10-05)
Revision as of 00:28, 27 October 2020 by RWSZ (Talk | contribs)


ssDNA, aptamer for α-amanitin(Best 2)

Another aptamer of alpha amatoxin selected by SELEX which has a proved better ability of binding with its target. It is the optimized version by elimination of 10bp from BB3953000, with higher enrichment coefficient. Better result of ELONA and dot blot further reinforces its optimization.

Background

Sequence and features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Characterisation of this part

Design

Experiment data

References

Usage and Biology

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Categories
Parameters
None