Coding

Part:BBa_K3645009:Design

Designed by: Huang nan   Group: iGEM20_Peking   (2020-10-26)
Revision as of 03:13, 27 October 2020 by Registry (Talk | contribs)

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PmCDA from Target-AID


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 671
    Illegal XhoI site found at 280
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is a fragment of original plasmid design, and contains other auxiliary parts (e.g. linkers, etc.)


Source

Banno S, Nishida K, Arazoe T, Mitsunobu H, Kondo A. Deaminase-mediated multiplex genome editing in Escherichia coli. Nat Microbiol. 2018 Apr;3(4):423-429. doi: 10.1038/s41564-017-0102-6. Epub 2018 Feb 5. PMID: 29403014.

References