Device

Part:BBa_K3629012

Designed by: Sravya Kakumanu   Group: iGEM20_Calgary   (2020-10-18)
Revision as of 04:42, 19 October 2020 by Sravyakakumanu (Talk | contribs) (→‎Design)


T. reesei CBHII expression construct with gibson homology sequences and FLAG tag


Usage and Biology

Design

The native signal peptide from T. reesei was removed so it would not interfere with the fused Lip2 secretion tag native to Y. lipolytica.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 2737
    Illegal EcoRI site found at 2561
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 2561
    Illegal NheI site found at 75
    Illegal SpeI site found at 2738
    Illegal PstI site found at 2752
    Illegal NotI site found at 7
    Illegal NotI site found at 2745
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
    Illegal EcoRI site found at 2561
    Illegal BamHI site found at 2686
    Illegal XhoI site found at 124
    Illegal XhoI site found at 1198
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 2738
    Illegal EcoRI site found at 2561
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal EcoRI site found at 2561
    Illegal XbaI site found at 16
    Illegal SpeI site found at 2738
    Illegal PstI site found at 2752
    Illegal NgoMIV site found at 1458
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None