Coding

Part:BBa_K3629009

Designed by: Sravya Kakumanu   Group: iGEM20_Calgary   (2020-10-12)
Revision as of 02:46, 19 October 2020 by Sravyakakumanu (Talk | contribs) (→‎Design)


Trichoderma reesei EGII with FLAG tag

Endoglucanase II coding sequence from Trichoderma reesei with FLAG tag.

Usage and Biology

Design

The native signal peptide from T. reesei was removed so it would not interfere with fused secretion tags native to Y. lipolytica

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 283
    Illegal NgoMIV site found at 817
    Illegal NgoMIV site found at 1135
  • 1000
    COMPATIBLE WITH RFC[1000]

References

[edit]
Categories
//awards/part_collection
//chassis/eukaryote/yeast
Parameters
None