Composite

Part:BBa_K3396017

Designed by: Yuxin Liu   Group: iGEM20_NUDT_CHINA   (2020-10-26)
Revision as of 00:00, 27 October 2020 by Registry (Talk | contribs)

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Replaceable1-Replaceable2-P2A-Truncated Trim21-Replaceable3

CMV->Replaceable1->linker->Replaceable2->P2A->Truncated Trim21->Replaceable3->BGH

This part is a composite part providing Golden Gate assembly based rapid construction of PREDATOR Pro plasmids for controlled degradation of specific target protein.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1106
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1063
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 840
    Illegal BsaI site found at 1789
    Illegal BsaI.rc site found at 786


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Categories
Parameters
None