Composite

Part:BBa_K3396016

Designed by: Changtai Xiao   Group: iGEM20_NUDT_CHINA   (2020-10-26)
Revision as of 23:38, 26 October 2020 by Registry (Talk | contribs)


CMV-replaceable-Fluc-P2A-Rluc

Dual luciferase reporter plasmid to indicate the target protein abundance. The coding sequence of target protein can be inserted into the replaceable region with Golden Gate assembly, the ratio Fluc/Rluc shall indicate the normalized protein amount of the target.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 871
    Illegal NgoMIV site found at 2215
    Illegal NgoMIV site found at 2236
    Illegal AgeI site found at 1939
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3102
    Illegal SapI.rc site found at 2121


[edit]
Categories
Parameters
None