Device

Part:BBa_K1682018:Design

Designed by: Nikola Dyulgyarov   Group: iGEM15_HKUST-Rice   (2015-09-17)
Revision as of 23:37, 26 October 2020 by Msindhu01824 (Talk | contribs) (→‎Design Notes)


PLTet0-1:NarL, PdcuS:sfGFP, J23109:TetR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2037
    Illegal NheI site found at 2060
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 318
    Illegal BglII site found at 486
    Illegal BamHI site found at 1995
    Illegal XhoI site found at 31
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1149


Design Notes

The PdcuS promoter was chosen for its NarL binding site, repressible activity, and the absence of other protein-binding domains, suggesting a robust, orthogonal response. The construct was assembled using Golden Gate Assembly. 

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Source

NarL, PdcuS - E. coli MG1655 genome sfGFP,TetR-PLTet0-1 scaffold - pRS334 (courtesy of Tabor Lab, Rice Univeristy)

References