Part:BBa_K3629002
Yarrowia lipolytica EXP Promoter
Promoter from the Yarrowia lipolytica EXP gene (an extracellular protease).
Usage and Biology
EXP promoter is a native Yarrowia lipolytica promoter for the EXP gene. Is one of the strongest constitutive promoters found in the organism. EXP promoter is often used for the expression of heterologous proteins in Y. lipolytica due to its high transcriptional level (1).
This promoter was used in the TrEGII expression construct (BBa_K3629017) as this promoter, in combination with the XPR2 signal peptide [1], has been shown to secrete high levels of TrEGII in Y. lipolytica (up to 132mg/L) (2). This promoter is thought not to be as strong as TEFin [2] promoter (2) but has approximately 1.2x greater activity than the wild-type TEF1 (BBa_K211700) promoter (2).
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 1
Illegal suffix found in sequence at 1025
Illegal SpeI site found at 602 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1
Illegal NheI site found at 663
Illegal SpeI site found at 602
Illegal SpeI site found at 1026
Illegal PstI site found at 1040
Illegal NotI site found at 7
Illegal NotI site found at 1033 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1
- 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 1
Illegal suffix found in sequence at 1026
Illegal SpeI site found at 602 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 1
Illegal XbaI site found at 16
Illegal SpeI site found at 602
Illegal SpeI site found at 1026
Illegal PstI site found at 1040 - 1000COMPATIBLE WITH RFC[1000]
References
1. Blazeck, J., Liu, L., Redden, H., & Alper, H. (2011). Tuning gene expression in Yarrowia lipolytica by a hybrid promoter approach. Applied and environmental microbiology, 77(22), 7905–7914. https://doi.org/10.1128/AEM.05763-11
2. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6333634/
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