Regulatory
Part:BBa_K3629002
Designed by: Sravya Kakumanu Group: iGEM20_Calgary (2020-10-11)
Revision as of 06:27, 26 October 2020 by ArshiaMostoufi (Talk | contribs)
Yarrowia lipolytica EXP Promoter
Promoter from the Yarrowia lipolytica EXP gene
Usage and Biology
EXP promoter is a native Yarrowia lipolytica promoter for the EXP gene. Is one of the strongest constitutive promoters found in the organism. EXP promoter is often used for the expression of heterologous proteins in Y. lipolytica due to its high transcriptional level (1).
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 1
Illegal suffix found in sequence at 1025
Illegal SpeI site found at 602 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1
Illegal NheI site found at 663
Illegal SpeI site found at 602
Illegal SpeI site found at 1026
Illegal PstI site found at 1040
Illegal NotI site found at 7
Illegal NotI site found at 1033 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1
- 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 1
Illegal suffix found in sequence at 1026
Illegal SpeI site found at 602 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 1
Illegal XbaI site found at 16
Illegal SpeI site found at 602
Illegal SpeI site found at 1026
Illegal PstI site found at 1040 - 1000COMPATIBLE WITH RFC[1000]
References
1. Blazeck, J., Liu, L., Redden, H., & Alper, H. (2011). Tuning gene expression in Yarrowia lipolytica by a hybrid promoter approach. Applied and environmental microbiology, 77(22), 7905–7914. https://doi.org/10.1128/AEM.05763-11
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Categories
Parameters
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