Part:BBa_K2924026
α-s1-casein
The milk protein α-s1-casein from Bos taurus
Usage and Biology
α-s1-casein (CSN1S1), also named B5B3R8_BOVIN1is a gene coding for a protein product and has the GeneID: 282208, which originates from
Caseins are among the most abundant proteins in native cow's milk. They are phosphoproteins and make up approximately 80% of total dairy protein mass. The milk proteins and their proportions are important to the parameters of milk manufacturing and the quality of milk protein.
With a length of 17508 bp and 19 exons the bovine α-s1-casein gene is the most abundant one of the bovine caseins (12-15 g/L)3. The α-s1-casein protein may contain up to eight serine monophosphate residues, which cluster in a hydrophilic domain between amino acids 43–80 and, through modelling studies, are thought to be connected to a hydrophobic domain (amino acids 100–199) by helical and sheet secondary structures4. In the ability of milk to carry calcium phosphate, α-s1-casein also performs an significant part5.
The DNA sequence of the gene was acquired by reverse-translating the amino acid sequence. Further, in order to reach optimal heterologous expression in Escherichia coli the DNA sequence was designed with optimized codons and synthesized commercially.
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Sequence and Features
[1]: https://www.uniprot.org/uniprot/B5B3R8
[2]: https://www.ncbi.nlm.nih.gov/gene/?term=282208
[3]: Boland, Mike, Harjinder Singh, and Abby Thompson, eds. Milk proteins: from expression to food. Academic Press, 2014.
[4]: Sheehy, P. A., et al. "Significance, origin and function of bovine milk proteins: the biological implications of manipulation or modification." Milk Proteins. Academic Press, 2008. 81-106.
[5]: Chanat, E., J. Cell Sci., 112, 3399-3412 (1999)
References
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