![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K3332054
Designed by: Yangqi Deng Group: iGEM20_XMU-China (2020-10-17)
J23100-RBS-GOX-AIDA-terminator
We anchored GOX protein onto membranes through AIDA to catalyze the reaction of degradating glyphosate to form glyoxalic acid and AMPA. We use K880005 to construct the expression system and anchor GOX on the surface of E.coli.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 355
Illegal BamHI site found at 2271 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 574
Illegal AgeI site found at 644
Illegal AgeI site found at 721 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |