![](https://parts.igem.org/images/partbypart/icon_coding.png)
Coding
Lpp-OmpA-
Part:BBa_K3332008
Designed by: Xiaoyu Wang Group: iGEM20_XMU-China (2020-10-17)
Lpp-OmpA-GOX
We anchor GOX protein onto membranes through Lpp-OmpA to catalyze the reaction of glyphosate to form glyoxalic acid and AMPA and add his-tag to purify the protein. We use K880005 to construct the expression system and anchor GOX on the surface of E.coli.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 759
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 978
Illegal AgeI site found at 1048
Illegal AgeI site found at 1125 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |