Coding
Lpp-OmpA-

Part:BBa_K3332008

Designed by: Xiaoyu Wang   Group: iGEM20_XMU-China   (2020-10-17)
Revision as of 20:16, 17 October 2020 by Registry (Talk | contribs)

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Lpp-OmpA-GOX

We anchor GOX protein onto membranes through Lpp-OmpA to catalyze the reaction of glyphosate to form glyoxalic acid and AMPA and add his-tag to purify the protein. We use K880005 to construct the expression system and anchor GOX on the surface of E.coli.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 759
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 978
    Illegal AgeI site found at 1048
    Illegal AgeI site found at 1125
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None