Composite

Part:BBa_K3332052

Designed by: Yangqi Deng   Group: iGEM20_XMU-China   (2020-10-17)
Revision as of 21:04, 17 October 2020 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


J23100-RBS-INPNC-GOX-terminator

We anchored GOX protein onto membranes through INPNC to catalyze the reaction of degradating glyphosate to form glyoxalic acid and AMPA. We use K880005 to construct the expression system and anchor GOX on the surface of E.coli.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1330
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 466
    Illegal NgoMIV site found at 1549
    Illegal AgeI site found at 1619
    Illegal AgeI site found at 1696
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None